| 产品编号 | bsm-60684R |
| 英文名称 | RUNX3 Recombinant Rabbit mAb |
| 中文名称 | Runx3重组兔单抗 |
| 别 名 | RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3(CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit(PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | R5G12 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RUNX3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Subcellular Location: Nucleus. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. SWISS: Q13761 Gene ID: 864 Database links: Entrez Gene: 864 Human
Omim: 600210 Human SwissProt: Q13761 Human Unigene: 170019 Human |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: MCF-7(Negative control), 2: Ramos,
3: Raji, 4: HT-1080
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 44-46 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
