| 产品编号 | bsm-60687R |
| 英文名称 | Cdc23 Recombinant Rabbit mAb |
| 中文名称 | 细胞分裂周期蛋白23重组兔单抗 |
| 别 名 | Cell division cycle protein 23 homolog; CDC23_HUMAN; APC8; CUT23; ANAPC8; Anaphase-promoting complex subunit 8(APC8); Cyclosome subunit 8; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | R4G5 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-2000 ICC=1:50-100 IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human CDC23: 510-575 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008] SWISS: Q9UJX2 Gene ID: 8697 Database links:
Entrez Gene: 8697 Human Entrez Gene: 52563 Mouse SwissProt: Q9UJX2 Human SwissProt: Q8BGZ4 Mouse
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| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HeLa, 2: K-562, 3: Mouse lung
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 69 kDa
Observed MW: 69 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
