| 产品编号 | bsm-60743R |
| 英文名称 | Chk1 Recombinant Rabbit mAb |
| 中文名称 | 细胞周期检测点激酶1重组兔单抗 |
| 别 名 | Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog(S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; CHK1 checkpoint homolog(S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A(S. cerevisiae); C85740; CHK1_HUMAN. |
| 研究领域 | 肿瘤 染色质和核信号 细胞周期蛋白 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 7D2 |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Chk1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.02% Proclin300 and 0.05% BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011]. SWISS: O14757 Gene ID: 1111 Database links: Entrez Gene: 1111 Human Entrez Gene: 12649 Mouse Omim: 603078 Human SwissProt: O14757 Human SwissProt: O35280 Mouse Unigene: 24529 Human Unigene: 16753 Mouse Unigene: 33267 Rat |
| 产品图片 |
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human U-2 OS cell lysates
Primary: Anti-Chk1 (bsm-60743R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
