| 产品编号 | bs-55145R |
| 英文名称 | NDUFB4 Rabbit pAb |
| 中文名称 | NDUFB4蛋白抗体 |
| 别 名 | B15; CI-B15; Complex I B15 subunit; Complex I-B15; NADH dehydrogenase(ubiquinone) 1 beta subcomplex 4 15kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; NADH ubiquinone oxidoreductase B15 subunit; NADH-ubiquinone oxidoreductase B15 subuni |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IF=1:50-100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human NDUFB4 protein: 1-90/129 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS with 0.02% Proclin300,50% Glycerol,pH7.3. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009] SWISS: O95168 Gene ID: 4710 |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human MOLT4 cell lysates
Lane 5: Human U87MG cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human U-2 OS cell lysates
Primary: Anti-NDUFB4 (bs-55145R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
