| 产品编号 | bsm-60759R |
| 英文名称 | STIP1 Recombinant Rabbit mAb |
| 中文名称 | 应激诱导磷蛋白1重组兔单抗 |
| 别 名 | Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; NY REN 11 antigen; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521. |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | B5C7 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 IP=1:50-100 IHC-P=1:100-500 Flow-Cyt=1:50-100 ICC=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STIP1 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). SWISS: P11831 Gene ID: 6722 |
| 产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human A549 cell lysates
Lane 5: Human A431 cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human Jurkat cell lysates
Primary: Anti-STIP1 (bsm-60759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Tissue: Human testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60759R
Tissue: Rat testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60759R
Cell line: Neuro-2a
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:500
Primary Ab incubation condition: 4°C
overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60759R
Cell line: HeLa
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:500
Primary Ab incubation condition: 4°C
overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60759R
Cell line: HeLa
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation
with primary antibody and secondary antibody(Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
