产品编号 | bsm-52660R |
英文名称 | CCNA2 Recombinant Rabbit mAb |
中文名称 | 周期素A2重组兔单抗 |
别 名 | CCNA2_HUMAN; Cyclin-A2; Cyclin-A; CCN1; CCN1; CCNA; cyclin A2; |
研究领域 | 肿瘤 心血管 细胞生物 细胞周期蛋白 |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | 2C2 |
交叉反应 | Human, (predicted: Mouse, Rat, ) |
产品应用 | WB=1:500-2000 IHC-P=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 49 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | A synthesized peptide derived from human Cyclin A2: 1-250 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016] SWISS: P51943 Gene ID: 12428 |
产品图片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-CCNA2 (bsm-52660R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |