| 产品编号 | bsm-52960R |
| 英文名称 | CRISPR-Cas9 Recombinant Rabbit mAb |
| 中文名称 | CRISPR-Cas9重组兔单抗 |
| 别 名 | CAS9_STRP1; CRISPR-associated endonuclease Cas9/Csn1; EC:3.1.-.-; SpCas9; SpyCas9; CRISPR-Cas9/Csn1; csn1; SPy_1046; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 6C1 |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IF=1:20-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 160 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Streptococcus pyogenes cas9 protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence. |
| 产品图片 |
Sample:
Lane 1: Recombinant CRISPR-Cas9 protein, C-His(bs-41498P)
Primary: Anti-CRISPR-Cas9 (bsm-52960R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 160 kDa
Observed band size: 182 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
