| 产品编号 | bsm-25439M |
| 英文名称 | Osteocalcin Mouse mAb |
| 中文名称 | 骨钙素单克隆抗体 |
| 别 名 | BGP; OC; OCN; Bglap2; Bgpr; Bgpra; OSTCN_BOVIN; BGLAP; Bone Gla protein (BGP); Gamma-carboxyglutamic acid-containing protein; OSTCN_HUMAN; OSTCN_PIG; OSTCN_RAT; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 8H8 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11 kDa |
| 检测分子量 | 25 |
| 性 状 | Lyophilized or Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BGP |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS (pH7.4). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Subcellular Location: Secreted. Post-translational modifications: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Similarity: Belongs to the osteocalcin/matrix Gla protein family.Contains 1 Gla (gamma-carboxy-glutamate) domain. SWISS: P02818 Gene ID: 632 Database links: Entrez Gene: 632 Human Omim: 112260 Human SwissProt: P02818 Human Unigene: 654541 Human Unigene: 9722 Rat 骨钙素又称骨γ-羧谷氨酸包含蛋白 |
| 产品图片 |
100 ng rhOsteocalcin-hFc protein (bs-43106P) per lane probed with Osteocalcin monoclonal antibody respectively, unconjugated (bsm-25439M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
