| 产品编号 | bsm-43143M |
| 英文名称 | PGC Mouse mAb |
| 中文名称 | 胃蛋白酶原C单克隆抗体 |
| 别 名 | PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 1A4 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | 40 |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 免 疫 原 | Recombinant human PGC protein: 17-388/388 |
| 亚 型 | IgG2b,κ |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009] Function: Hydrolyzes a variety of proteins. Subcellular Location: Secreted. Similarity: Belongs to the peptidase A1 family. SWISS: P20142 Gene ID: 5225 Database links: Entrez Gene: 5225 Human Omim: 169740 Human SwissProt: P20142 Human Unigene: 1867 Human Unigene: 9735 Rat 胃蛋白酶原C(PG-C)主要用于胃癌及其癌前病变易感性的关系。 |
| 产品图片 |
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-PGC (bsm-43143M) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 42 kDa
Observed band size: 39 kDa
Sample:
Lane 1: Recombinant human PGC protein, C-His(bs-43143P)
Primary: Anti-PGC (bsm-43143M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-43143M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
