| 产品编号 | bsm-60790M |
| 英文名称 | CD57 Mouse mAb |
| 中文名称 | 髓鞘相关糖蛋白（CD57）单克隆抗体 |
| 别 名 | CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1; B3GA1_HUMAN; B3GAT1; Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P (GlcAT-P); UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase (GlcUAT-P); 2.4.1.135; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | G14H3 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD57 |
| 亚 型 | IgG1, k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] Function: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Subunit: Homodimer (Potential). Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Tissue Specificity: Mainly expressed in the brain. Similarity: Belongs to the glycosyltransferase 43 family. SWISS: Q9P2W7 Gene ID: 27087 Database links: Entrez Gene: 27087 Human Omim: 151290 Human SwissProt: Q9P2W7 Human Unigene: 381050 Human CD57是一种分子量为110KD的糖蛋白,它是自然杀伤细胞(NK)和杀伤细胞的特异性表面抗原。CD57表达于15-20%的外周血单核细胞，60%的NK活化细胞以及T细胞亚群。该抗体可以标记NK细胞，也可识别非淋巴组织的一些细胞。可用于NK细胞介导的细胞毒、NK细胞及T细胞亚群的功能等方面的研究，也可标记神经内分泌细胞及其肿瘤。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated(bsm-60790M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
