| 产品编号 | bsm-0474M |
| 英文名称 | human IgA Mouse mAb |
| 中文名称 | 人血清型免疫球蛋白A抗体 |
| 别 名 | Native Human IgA protein; Human IgA protein; IgA1; Igh2; IGHA 1; IGHA 2; IGHA1; IGHA2; Immunoglobulin alpha 1; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin heavy chain 2(serum IgA); Immunoglobulin heavy constant alpha 1; Immunoglobulin heavy constant alpha 2; Human Immunoglobulin A; human Serum IgA; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7H8 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 免 疫 原 | purified human Serum IgA |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. Subcellular Location: Secreted. SWISS: P01876 Gene ID: 3493 IgA有IgA1和IgA2两个亚类。IgA1主要存在于血清中，约占血清中IgA的85%，α1链分子量为56kD；IgA2主要存在于外分泌液中，少部分以血清型IgA存在，约占血清中IgA的15%，α2链缺乏铰链区，分子量为52kD。血清中的IgA除单体形式外还有由J链共价相连的二聚体或三聚体等形式。分泌型IgA是由J连接的双体和分泌成分所组成，主要存在于初乳、唾液、泪液、胃肠液、支气管分泌等外分泌液中，是粘膜局部免疫的最重要因素，分泌型IgA通过与相应的病原微生物（如脊髓灰质炎病毒）结合，阻抑其吸附到易感细胞上，分泌型IgA还可中和毒素如霍乱弧菌毒素和大肠杆毒素等。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human IgA ) Monoclonal Antibody, Unconjugated (bsm-0474M) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human IgA) Monoclonal Antibody, Unconjugated (bsm-0474M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
