| 产品编号 | bsm-42173M |
| 英文名称 | FOXP3 Mouse mAb |
| 中文名称 | 叉头蛋白P3单克隆抗体 |
| 别 名 | Forkhead box protein P3; forkhead box P3; Scurfin; Forkhead box protein P3, C-terminally processed; Forkhead box protein P3 41 kDa form; JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3_HUMAN; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2H5 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 免 疫 原 | Recombinant human FOXP3 protein: 1-193/431 |
| 亚 型 | Mouse Ig1, κ |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. Function: Probable transcription factor. Plays a critical role in the control of immune response. Subunit: Interacts with IKZF3. Subcellular Location: Nucleus (Potential). Post-translational modifications: Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. DISEASE: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SWISS: Q9BZS1 Gene ID: 50943 Database links: Entrez Gene: 50943 Human Entrez Gene: 20371 Mouse Omim: 300292 Human SwissProt: Q9BZS1 Human SwissProt: Q99JB6 Mouse Unigene: 247700 Human Unigene: 182291 Mouse |
| 产品图片 |
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-FOXP3 antibody (bsm-42173M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Foxp3 antibody (bsm-42173M): 2μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-40296G-FITC): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
