| 产品编号 | bsm-60846M |
| 英文名称 | ASNS Mouse mAb |
| 中文名称 | 天冬酰胺合成酶单克隆抗体 |
| 别 名 | ASNSD; TS11; ASNS_HUMAN; ASNS; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; 6.3.5.4; ASNS_MOUSE; ASNS_RAT; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ASNS |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] DISEASE: Asparagine synthetase deficiency (ASNSD) [MIM:615574]: An inborn error of asparagine biosynthesis that results in a severe neurologic disorder characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, cortical atrophy, and seizure or hyperekplexic activity. {ECO:0000269|PubMed:24139043}. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain. SWISS: P08243 Gene ID: 440 Database links: Entrez Gene: 440 Human Entrez Gene: 27053 Mouse SwissProt: P08243 Human SwissProt: Q61024 Mouse |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with ASNS monoclonal antibody, unconjugated (bsm-60846M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (bsm-60846M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (bsm-60846M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (bsm-60846M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (bsm-60846M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (bsm-60846M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
