| 产品编号 | bsm-43036M |
| 英文名称 | SIGLEC15 Mouse mAb |
| 中文名称 | 唾液酸结合性免疫球蛋白样凝集素15单克隆抗体 |
| 别 名 | CD33 antigen-like 3; CD33 molecule-like 3; CD33L3; HsT1361; Sialic acid-binding Ig-like lectin 15; SIG15_HUMAN; Siglec-15; SIGLEC15. |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 免 疫 原 | Recombinant human SIGLEC15 protein |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated Siglecs or sialoadhesins, recognize sialylated ligands and play a key role in mediating sialic-acid dependent binding to cells. Siglec-15 (sialic acid binding Ig-like lectin 15), also known as CD33L3, is a 328 amino acid single-pass type I membrane protein that contains one Ig-like C2-type domain and one Ig-like V-type domain. Expressed in dendritic and macrophage cells, Sinlec-15 interacts with DAP10 and DAP12 and binds to sialylated glycoproteins. The gene encoding Siglec-15 maps to human chromosome 18, which houses over 300 protein-coding genes and contains nearly 76 million bases. Function: Binds sialylated glycoproteins. Subcellular Location: Membrane. Tissue Specificity: Expressed in macrophage and/or dendritic cells of spleen and lymph nodes. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q6ZMC9 Gene ID: 284266 Database links: Entrez Gene: 284266 Human SwissProt: Q6ZMC9 Human Unigene: 287692 Human |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human U87MG cell lysates
Primary: Anti-SIGLEC15 (bsm-43036M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
