产品编号 | bsm-60864M |
英文名称 | Benzoyllysine Mouse mAb |
中文名称 | 苯甲酰基赖氨酸/苯甲酰化赖氨酸单克隆抗体 |
别 名 | Benzoylated Lysine; Benzoyllysine; benzyl-lysine; benzyl Lys; Kbz; 赖氨酸苯甲酰化; 苯甲酰化修饰赖氨酸 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 9B7 |
交叉反应 | Human, Mouse, Rat, |
产品应用 | WB=1:500-1000 IHC-P=1:200-1000 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | kDa |
检测分子量 | |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | benzoylated BSA |
亚 型 | IgG |
纯化方法 | 免疫亲和层析纯化 |
缓 冲 液 | PBS, Glycerol, BSA. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Lysine benzoyllysine is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine benzoylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes. 赖氨酸苯甲酰化是一种进化保守的修饰,在很多原核和真核生物中都有表达。 |
产品图片 |
Sealing solution and concentration: 5% NFDM/TBST
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti Mouse IgG H&L (HRP)
Cracking liquid: HeLa, N2a, HepG2
Protein loading amount: 20 μ G
Exposure time: 60 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa
Tissue: human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: human testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: rat brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |