| 产品编号 | bsm-60866R |
| 英文名称 | Citrulline-Histone H3 (Arg2/8/17) Recombinant Rabbit mAb |
| 中文名称 | 瓜氨酸组蛋白3重组兔单抗 |
| 别 名 | H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD; H3R2/R8/R17cit; 瓜氨酸化组蛋白3; 瓜氨酸组蛋白H3; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. 产品组分及规格 编号 组分 规格 浓度 储存 1 |
| 产品图片 |
Sealing solution and concentration: 5% NFDM/TBST
Dilution of primary antibody: 1:2000
Incubation conditions for primary antibody: 2 hours at room temperature
Immunogen peptide sample size: 1:1 ng, 4 ng, 16 ng, 64 ng, 2-6:64ng
Exposure time: 10 seconds
Sealing solution and concentration: 5% NFDM/TBST
Dilution of primary antibody: 1:20000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP)
Cracking solution: (-): HL-60, (+): HL-60 + DMSO + A23187 (4uM,15min)
Protein loading amount: 20 μ g
Exposure time: 60 seconds
Theoretical molecular weight: 15 kDa
Actual molecular weight: 15 kDa
Sealing solution and concentration: 5% NFDM/TBST
Dilution ratio of primary antibody: 1:100000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP)
Cracking solution: (Lane 1) 293T transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate; (Lane 2) 293T transfected with GFP-tagged PADI4 (WT, Q9UM07) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.
Protein loading amount: 20 μ g
Exposure time: 10 seconds
Theoretical molecular weight: 15 kDa
Actual molecular weight: 15 kDa
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Antibody incubation with Citrulline-Histone H3 (Arg2/8/17) Monoclonal Antibody, Unconjugated(bsm-60866R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Negative control： site-specific peptide blocking
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
