| 产品编号 | bsm-55523R |
| 英文名称 | MUC1/CD227 Recombinant Rabbit mAb |
| 中文名称 | 粘蛋白-1重组兔单抗 |
| 别 名 | ADMCKD; ADMCKD1; CD227; MCD; MCKD; MCKD1; PEM; PUM; PEMT; Episialin; EAM; CA 15-3 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | SB48 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 免 疫 原 | Synthesized peptide derived from Human MUC1 |
| 亚 型 | IgG |
| 纯化方法 | Affinity Purification |
| 缓 冲 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存条件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation . MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail. MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation. MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail. Overexpression of MUC1 potentiates transformation and attenuates stress-induced apoptosis through the Akt or p53 pathways SWISS: P15941 Gene ID: 4582 Mucin（CD227）是糖蛋白家族的一员，以重复结构域和密集的O-糖基化为特征。MUC1在呼吸道、胃肠道等所有上皮组织、皮脂腺和唾液腺以及造血细胞中都有表达，MUC1的非上皮性表达已被描述发生在白细胞上，包括T细胞、B细胞、骨髓单核细胞、单核细胞和树突状细胞（DCs）等。MUC1可进行蛋白质水解裂解，并且较大的胞外域与较小的羧基末端结构域有关，后者包含一个跨膜片段和一个含72个残基的胞质尾区。MUC1对正常的上皮起润滑和保护作用，同时还介导信号转导和细胞黏附功能，免疫活化和抑制作用等。在肿瘤组织中，MU |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (bsm-55523R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
