| 产品编号 | IHC0318H |
| 英文名称 | Human AIF Ready-To-Use IHC Kit |
| 中文名称 | 人凋亡诱导因子即用型免疫组化试剂盒 |
| 别 名 | AIF, AIFM1, AIFsh2, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, Hq, NADMR, NAMSD, PDCD8 |
| 适用样品基质 | FFPE tissue |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 产品介绍 |
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10. SWISS: O95831 Gene ID: 9131 |
| 产品图片 |
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human colon cancer tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human testis tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human liver tissue slide using IHC0318H (Human AIF Kit).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
