| 产品编号 | IHC0340H |
| 英文名称 | Human Shh Ready-To-Use IHC Kit |
| 中文名称 | 人Shh信号转导通路膜蛋白受体即用型免疫组化试剂盒 |
| 别 名 | 9530036O11Rik, Dsh, HHG1, HLP3, HPE3, Hx, Hxl3, M100081, MCOPCB5, SHH, SMMCI, TPT, TPTPS, Vhh-1 |
| 适用样品基质 | FFPE tissue |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 产品介绍 |
Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. SWISS: Q15465 Gene ID: 6469 |
| 产品图片 |
Immunohistochemical analysis of paraffin embedded human pancreatic cancer tissue slide using IHC0340H (Human Shh Kit).
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0340H (Human Shh Kit).
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma tissue slide using IHC0340H (Human Shh Kit).
Immunohistochemical analysis of paraffin embedded human liver tissue slide using IHC0340H (Human Shh Kit).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
