| 产品编号 | bsm-54409R |
| 英文名称 | Carbonic Anhydrase I Recombinant Rabbit mAb |
| 中文名称 | 碳酸酐酶1重组兔单抗 |
| 别 名 | Carbonic Anhydrase-I; Carbonic Anhydrase 1; Carbonic anhydrase1; Carbonic anhydrase-1; JW0122; CA 1; CA1; CA I; CA-1; CAI; CAN; Car 1; Car1; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase B; Carbonic dehydratase; ECK0125; yadF; CAH1_HUMAN. |
| 研究领域 | 心血管 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 2H4 |
| 交叉反应 | Mouse, Rat, (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Carbonic Anhydrase I: 1-200/261 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009] Function: Reversible hydration of carbon dioxide. Subcellular Location: Cytoplasm. Similarity: Belongs to the alpha-carbonic anhydrase family. SWISS: P00915 Gene ID: 759 Database links: Entrez Gene: 759 Human Entrez Gene: 12346 Mouse Omim: 114800 Human SwissProt: P00915 Human SwissProt: P13634 Mouse Unigene: 23118 Human Unigene: 273195 Mouse Unigene: 6854 Rat |
| 产品图片 |
Sample:
Lane 1: Large intestine (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Stomach (Mouse) Lysate at 40 ug
Lane 4: Bone (Mouse) Lysate at 40 ug
Lane 5: Spleen (Mouse) Lysate at 40 ug
Lane 6: Small intestine (Mouse) Lysate at 40 ug
Lane 7: Lung (Mouse) Lysate at 40 ug
Lane 8: Cerebrum (Mouse) Lysate at 40 ug
Lane 9: Large intestine (Rat) Lysate at 40 ug
Lane 10: Lung (Rat) Lysate at 40 ug
Lane 11: Stomach (Rat) Lysate at 40 ug
Lane 12: Bone (Rat) Lysate at 40 ug
Lane 5: Spleen (Rat) Lysate at 40 ug
Primary: Anti-Carbonic Anhydrase I (bsm-54409R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 27 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
