| 产品编号 | bs-101749P |
| 英文名称 | Recombinant Human MDM2 Protein, N-His |
| 中文名称 | |
| 别 名 | MDM2; Hdm2; p53-binding protein Mdm2; E3 ubiquitin-protein ligase Mdm2; RING-type E3 ubiquitin transferase Mdm2; Double minute 2 protein; Oncoprotein Mdm2 |
| 克 隆 号 | |
| 理论分子量 | 57.31 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Inhibits TP53/p53- and TP73/p73-mediated cell cyclearrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein(RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with TP53/p53 and WWOX. Interacts with CDKN2AIP, MTBP, TRBG1 and USP7. Isoform Mdm2-F does not interact with TP53/p53. Interacts with PYHIN1. Interacts with, and ubiquitinates HIV-1 Tat. Belongs to the MDM2/MDM4 family. SWISS: Q00987 Gene ID: 4193 Mdm2基因在肿瘤发生、发展的过程中起着重要作用，Mdm2蛋白过度表达与p53基因突变有重要的关联性。Mdm2基因在肿瘤发生、发展的过程中起着重要作用。MDM2广泛分布于人体正常组织中，可与p53蛋白相互作用。活化的p53导致MDM2表达，而MDM2的表达又可抑制野生型p53的转录激活作用。MDM2通常在软组织肉瘤中有过表达现象，而在膀胱癌、乳腺癌、宫颈癌、胶质细胞瘤中也有一定程度的表达，并可能与肿瘤细胞的耐药有关。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
