| 产品编号 | bs-101864P |
| 英文名称 | Recombinant Human HYAL1 Protein, N-His |
| 中文名称 | |
| 别 名 | LuCa-1; HYAL1; Hyaluronidase-1; Hyal-1; LUCA1; Lung carcinoma protein 1; Hyaluronoglucosaminidase-1 |
| 克 隆 号 | |
| 理论分子量 | 48.45 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis. SWISS: Q12794 Gene ID: 3373 HAase是一种具有高度底物特异性的蛋白酶，使透明质酸产生低分子化作用的酶之总称,可以特异地作用于透明质酸，通过裂解葡萄糖胺和葡萄糖醛酸的分子键而将透明质酸分解为二糖成分，引起透明质酸的解聚和降解，透明质酸分子从胶原细纤维间隙中释放出来并重新分布，主要定位于细胞外基质影响细胞的增殖、分化及迁移,并在胚胎发育和肿瘤发生发展过程中发挥作用,因而它在胚胎发育、血管形成、免疫监视以及肿瘤发生发展过程中起着重要作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
