| 产品编号 | bs-101925P |
| 英文名称 | Recombinant Human ILK Protein, N-His |
| 中文名称 | |
| 别 名 | ILK; ILK2; ILK1; ILK-2; ILK-1; 59 kDa serine/threonine-protein kinase; Beta-integrin-linked kinase; p59ILK; Integrin-linked protein kinase |
| 克 隆 号 | |
| 理论分子量 | 33.14 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active. SWISS: Q13418 Gene ID: 3611 ILK 是一种新发现的Ser/Thr蛋白激酶。ILK能够通过与整合素β1亚单位的结合介导细胞与胞外基质的连接，以依赖于PI3K的方式激活，并通过磷酸化下游底物PKB/AKT,GSK3等胞外信号的一项下游传递，对细胞的生长，分化，迁移等进行调控。由于ILK在胞内外信号传导中起着重要的作用。并且抑制ILK的活性能够导致细胞周期的停滞和细胞程序性死亡的启动，使其成为肿瘤治疗和肿瘤药物的理想靶位点。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
