| 产品编号 | bs-102197P |
| 英文名称 | Recombinant Human CD272/BTLA Protein, C-His |
| 中文名称 | |
| 别 名 | B- and T-lymphocyte attenuator; BTLA; CD272; B- and T-lymphocyte-associated protein |
| 克 隆 号 | |
| 理论分子量 | 18.18 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >85% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
B and T lymphocyte attenuator (BTLA), an immunoglobulin domain-containing glycoprotein with two immunoreceptor tyrosine-based inhibitory motifs. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. Crosslinking BTLA with antigen receptors induces its tyrosine phosphorylation and association with the Src homology domain 2 (SH2)-containing protein tyrosine phosphatases SHP-1 and SHP-2, and attenuates production of interleukin 2 (IL-2). BTLA-deficient T cells show increased proliferation, and BTLA-deficient mice have increased specific antibody responses and enhanced sensitivity to experimental autoimmune encephalomyelitis. B7x, a peripheral homolog of B7, is a ligand of BTLA. Thus, BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1). SWISS: Q7Z6A9 Gene ID: 151888 BTLA对T细胞的活化、增殖起着重要的负调控作用，BTLA相应配体为TNFR超家族中的疱疹病毒入侵介质（HVEM）, 其表达于包括T细胞在内的多种免疫细胞表面。 有学者将他定为CD28的超级族成员，B、T淋巴细胞衰减子-CD272主要用于细胞信号传导方面的研究。 近来国外学者对BTLA用于抑制肿瘤方面的研究也有了新的进展，认为B、T淋巴细胞衰减子对肿瘤的生长有抑制作用，探索新的肿瘤免疫治疗有了新的途径， 封闭此途径有可能成为肿瘤免疫治疗的新靶点。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
