| 产品编号 | bsm-43705M |
| 英文名称 | CD45RA Mouse mAb |
| 中文名称 | CD45RA单克隆抗体 |
| 别 名 | B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200; Ly-5; Lyt-4; loc; RT7; PTPRC_HUMAN; PTPRC; Leukocyte common antigen (L-CA); 3.1.3.48; PTPRC_MOUSE; Lymphocyte antigen 5 (Ly-5); PTPRC_RAT; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 5C9 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 138/148 kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD45RA protein: 26-482/1121 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling SWISS: P08575 Gene ID: 5788 CD45在活化信号转导中起到调节作用，在确定CD45为一种PTPase之前就已证实了CD45参于细胞的活化和生长调节。 抗CD45抗体可以抑制PHA或CD3交联所介导的T细胞增殖，还可抑制NK或细胞毒性T细胞对靶细胞的杀伤，抑制经CD2、CD3以及CD8膜分子介导的信号转导作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
