| 产品编号 | bsm-55660M |
| 英文名称 | GPX4 Mouse mAb |
| 中文名称 | 谷胱甘肽过氧化酶4单克隆抗体 |
| 别 名 | GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx; mtPHGPx; GPX4_HUMAN; GPX4; Glutathione peroxidase 4 (GPx-4 | GSHPx-4); 1.11.1.12; GPX4_MOUSE; GPX4_RAT; glutathione peroxidase 4; glutathione peroxidase 4 (phospholipid hydroperoxidase); phospholipid hydroperoxidase; selenoprotein GPX4 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 检测分子量 | 20 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse GPX4 protein |
| 亚 型 | IgG2a, κ |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped aShipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.t 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site. SWISS: O70325 Gene ID: 625249 谷胱甘肽过氧化物酶（GSH-Px）是机体内广泛存在的一种重要的过氧化物分解酶。硒是GSH-Px酶系的组成成分，它能催化GSH变为GSSG，使有毒的过氧化物还原成无毒的羟基化合物，同时促进H2O2的分解，从而保护细胞膜的结构及功能不受过氧化物的干扰及损害。GSH-Px的活性中心是硒半胱氨酸，其活力大小可以反映机体硒水平。GSH-Px酶系主要包括4种不同的GSH-Px，分别为胞浆GSH-Px、血浆GSH-Px、磷脂氢过氧化物GSH-Px及胃肠道专属性GSH-Px。胞浆GSH-Px由4个相同的分子量大小为22k |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
