| 产品编号 | bs-6070R-BF594 |
| 英文名称 | Anti-phospho-LLGL1 + LLGL2(Ser650+Ser654)/BF594 |
| 中文名称 | BF594标记的磷酸化相似肿瘤抑制基因HUGL抗体 |
| 别 名 | LLGL1 + LLGL2 (phospho S650 + S654); LLGL1 + LLGL2 (phospho Ser650 + Ser654); p-LLGL1 + p-LLGL2 (S650 + S654); p-LLGL1/LLGL2 (Ser650 + Ser654); p-LLGL1/LLGL2 (S650 + S654); p-LLGL1/LLGL2 (Ser650/654); DLG4; HGL; HUGL; HUGL1; lethal giant larvae homolog 1; lethal giant larvae homolog 2; Lethal(2) giant larvae protein homolog 1; Lethal(2) giant larvae protein homolog 2; LGL2; LLGL; L2GL1_HUMAN; L2GL2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞周期蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Guinea Pig, k) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 117kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from mouse LLGL1 around the phosphorylation site of Ser650+Ser654 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17. LLGL2 is a protein similar to lethal giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Function: Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Subunit: Associated with nonmuscle myosin II heavy chain. Interacts with PRKCI/aPKC, PARD6B/Par-6 and PARD6A. Interacts with STX4A. Subcellular Location: Cytoplasm, cytoskeleton. Note=Localized to the lateral membrane during the polarization and formation cell-cell contacts. Tissue Specificity: Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated at least at Ser-663 by PRKCI. Similarity: Belongs to the WD repeat L(2)GL family. Contains 14 WD repeats. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
