| 产品编号 | bs-2803R-Cy3 |
| 英文名称 | Anti-TNFAIP3/Cy3 |
| 中文名称 | Cy3标记的肿瘤坏死因子α诱导蛋白3抗体 |
| 别 名 | Zinc finger protein A20; A20; TNFA1P2; TNFAIP 3; Tnfip 3; Tnfip3; Tumor necrosis factor alpha induced protein 3; Tumor necrosis factor induced protein 3; Tumor necrosis factor inducible protein A20; MGC104522; MGC138687; MGC138688; OTU domain containing protein 7C; OTUD7C; Putative DNA binding protein A20. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 90kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TNFAIP3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases Function: TNFAIP3 interacts with NAF1 and inhibits TNF induced NF kappa B dependent gene expression by interfering with a RIP or TRAF2 mediated transactivation signal. It inhibits programmed cell death, has a role in the function of the lymphoid system, and may contribute to the in vivo effects of TNF. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the peptidase C64 family. Contains 7 A20-type zinc fingers. Contains 1 OTU domain. Database links: Entrez Gene: 7128 Human Entrez Gene: 21929 Mouse Omim: 191163 Human SwissProt: P21580 Human SwissProt: Q60769 Mouse Unigene: 211600 Human Unigene: 116683 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. TNFAIP3是一种泛素修饰酶，是机体重要的炎症反应负性调节因子,也是NF-κB的负调节蛋白。TNFAIP3蛋白与很多变态反应疾病的病理生理演变相关联，如：包括类风湿关节炎、冠状动脉疾病、银屑病、腹腔疾病、I型糖尿病、炎症性肠炎和系统性红斑狼疮等。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
