| 产品编号 | bs-3523R-PE-Cy7 |
| 英文名称 | Anti-PBK/PE-Cy7 |
| 中文名称 | PE-Cy7标记的PDZ连接激酶/T-LAK细胞源蛋白激酶抗体 |
| 别 名 | TOPK; Cancer/testis antigen 84; CT84; FLJ14385; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; PDZ-binding-kinase/T-LAK cell-originated protein kinase； Nori 3; Nori3; PDZ binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; SPK; T LAK cell originated protein kinase; TOPK. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PBK |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Subunit: Interacts with DLG1 and TP53. Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Post-translational modifications: Phosphorylated; in a cell-cycle dependent manner at mitosis. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 55872 Human Entrez Gene: 52033 Mouse Omim: 611210 Human SwissProt: Q96KB5 Human SwissProt: Q9JJ78 Mouse Unigene: 104741 Human Unigene: 24337 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PBK/TOPK是一种新近发现的丝-苏氨酸激酶,属于MAPKK分子家族成员，具有参与调控恶性肿瘤细胞的增殖和周期变化, 促进肿瘤细胞转化, 并且通过MAPKK信号通路参与调控细胞DNA损伤修复. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
