| 产品编号 | bs-7665R-AP |
| 英文名称 | Anti-NUP50/AP |
| 中文名称 | 碱性磷酸酶（AP）标记的核孔蛋白50抗体 |
| 别 名 | NPAP60; NPAP60L; Nuclear pore-associated protein 60 kDa-like; nucleoporin 50; NUP50_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, ) |
| 产品应用 | IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUP50 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: NUP50 is a component of the nuclear pore complex that has a direct role in nuclear protein export. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. NUP50 interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1. Function: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. Subunit: Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Subcellular Location: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity). Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity). Tissue Specificity: Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. Similarity: Contains 1 RanBD1 domain. Database links: Entrez Gene: 10762 Human Entrez Gene: 18141 Mouse Omim: 604646 Human SwissProt: Q9UKX7 Human SwissProt: Q9JIH2 Mouse Unigene: 727643 Human Unigene: 28379 Mouse Unigene: 391486 Mouse Unigene: 123156 Rat Unigene: 3242 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
