| 产品编号 | bs-7717R-Gold |
| 英文名称 | Anti-CDCA5/Gold |
| 中文名称 | 胶体金标记的细胞分裂周期相关蛋白5抗体 |
| 别 名 | Cell division cycle associated protein 5; MGC16386; p35; Sororin; CDCA5_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 细胞生物 染色质和核信号 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDCA5 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: Sororin, also designated cell division cycle-associated protein 5 (CDCA5) or p35, functions as a regulator of sister chromatid cohesion during mitosis. It interacts with the APC/C complex and is found in a complex consisting of cohesion components SCC-112, MC1L1, SMC3L1, RAD21 and APRIN. The deduced human and mouse Sororin proteins consist of 252 and 264 amino acid residues, respectively, and both contain a KEN box for APC-dependent ubiquitination. Reserach demonstrates a punctated nuclear distribution of Sororin during interphase and a diffuse distribution throughout the cell during mitosis. There is no apparent concentration of Sororin on chromatin in mitotic cells and Sororin levels decrease in synchronized HeLa cells during interphase. The Sororin gene maps to chromosome 11q12.1. Function: CDCA5 is a regulator of sister chromatid cohesion in mitosis. It may act by regulating the ability of the cohesin complex to mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with the chromosomes. CDCA5 interacts with the APC/C complex and is found in a complex with the cohesin components SMC1L1, SMC3L1, RAD21, PDS5A/APRIN and PDS5B/SCC-112. Subunit: Interacts with the APC/C complex (By similarity). Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPAL. Subcellular Location: Nuclear. Post-translational modifications: Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5. Ubiquitinated by the APC/C complex in G1, leading to its degradation (Probable). Similarity: Belongs to the sororin family. Database links: UniProtKB/Swiss-Prot: Q96FF9.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
