| 产品编号 | bs-0190R-BF647 |
| 英文名称 | Anti-PACAP-27/38/BF647 |
| 中文名称 | BF647标记的腺苷酸环化酶激活肽-27/38抗体 |
| 别 名 | Adenylate Cyclase Activating Polypeptide 1 (Pituitary); Prepro-PACAP; PACAP; Pituitary Adenylate Cyclase Activating Polypeptide; PRP-48; PACAP27; PACAP38; ADCYAP1; PACA_HUMAN; |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACAP-27/38 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013] Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity). Subunit: Interacts with ADCYAP1R1 (via N-terminal extracellular domain). Subcellular Location: Extracellular region or secreted Similarity: Belongs to the glucagon family. Database links: Entrez Gene: 116 Human Entrez Gene: 11516 Mouse Omim: 102980 Human SwissProt: P18509 Human SwissProt: O70176 Mouse Unigene: 531719 Human Unigene: 727476 Human Unigene: 3407 Mouse Unigene: 37400 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 腺苷酸环化酶激活肽是一种可激活垂体腺苷酸环化酶的多肽，有PACAP-27和 PACAP-38两种形式，他们共同具有N端27个氨基酸，属于PACAP/glucagon超级家族。在中枢神经系统分布广泛，其中以下丘脑含量最高。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
