| 产品编号 | bs-1203R-Gold |
| 英文名称 | Anti-TOP2A/Gold |
| 中文名称 | 胶体金标记的DNA拓普西异构酶Ⅱ抗体 |
| 别 名 | DNA topoisomeraseⅡalpha; TOPO II; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; TOP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN; Topo IIα; Topo-IIα. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 肿瘤 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 174kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TOPO II alpha |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq]. Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. Subunit: Homodimer. Interacts with COPS5. Subcellular Location: Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm. Post-translational modifications: Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. Similarity: Belongs to the type II topoisomerase family. Contains 1 Toprim domain. Database links: Entrez Gene: 7153 Human Entrez Gene: 21973 Mouse Omim: 126430 Human SwissProt: P11388 Human SwissProt: Q01320 Mouse Unigene: 156346 Human Unigene: 4237 Mouse Unigene: 90996 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 拓扑异构酶II是一种能催化DNA 拓扑异构体相互变换，调节DNA三维结构的核酶。与染色质或染色体的组装与分组装、基因组复制、DNA重组、基因转录及DNA损伤后修复等有密切的关系。许多人的恶性肿瘤都有拓扑异构酶II的过表达，因此，拓扑异构酶II是许多化疗药的靶标。大部分以拓扑异构酶II为重要靶标抗肿瘤的化疗药物是通过稳定的DNA与拓扑异构酶II的复合物激发凋亡的。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
