| 产品编号 | bs-5547R-Gold |
| 英文名称 | Anti-Phospho-PLCG 2 (Tyr753)/Gold |
| 中文名称 | 胶体金标记的磷酸化磷酯酶Cγ2抗体 |
| 别 名 | PLCG 2 (phospho Y753); p-PLCG 2 (phospho Y753); PLCG2(phospho Y753); PLCG 2(Phospho Tyr753); PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 3.1.4.11; Phosphoinositide phospholipase C; Phospholipase C gamma 2; Phospholipase C, gamma 2 (phosphatidylinositol specific). |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 139kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr753 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3. Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. Subunit: Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Post-translational modifications: Phosphorylated on tyrosine residues by CSF1R. Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity. DISEASE: Defects in PLCG2 are the cause of familial cold autoinflammatory syndrome type 3 (FCAS3) [MIM:614468]. An autosomal dominant immune disorder characterized by the development of cutaneous urticaria, erythema, and pruritis in response to cold exposure. Affected individuals have variable additional immunologic defects, including antibody deficiency, decreased numbers of B cells, defective B cells, increased susceptibility to infection, and increased risk of autoimmune disorders. Similarity: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain. Database links: Entrez Gene: 5336 Human Entrez Gene: 234779 Mouse Omim: 600220 Human SwissProt: P16885 Human SwissProt: Q8CIH5 Mouse Unigene: 413111 Human Unigene: 192699 Mouse Unigene: 9751 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
