| 产品编号 | bs-4201R-Gold |
| 英文名称 | Anti-FIH1/HIF1AN/Gold |
| 中文名称 | 胶体金标记的缺氧诱导因子1α抑制蛋白/HIF1AN抗体 |
| 别 名 | Hypoxia inducible factor asparagine hydroxylase; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Peptide aspartate beta dioxygenase; HIF1AN; DKFZp762F1811; FIH1; HIF1AN; FLJ20615; FLJ22027; HIF1N_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 染色质和核信号 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 38kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FIH1/HIF1AN |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: HIF1AN (Hypoxia inducible factor 1 alpha inhibitor) is a corepressor that interacts with hypoxia inducible factor 1 (HIF1) alpha and the von Hippel Lindau tumor suppressor protein to mediate repression of HIF1 transcriptional activity. Function: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminaltransactivation domain (CAD). Functions as an oxygen sensor and,under normoxic conditions, the hydroxylation prevents interactionof HIF-1 with transcriptional coactivators includingCbp/p300-interacting transactivator. Involved in transcriptionalrepression through interaction with HIF1A, VHL and histonedeacetylases. Hydroxylates specific Asn residues within ankyrinrepeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A andseveral other ARD-containing proteins. Also hydroxylates Asp andHis residues within ARDs of ANK1 and TNKS2, respectively.Negatively regulates NOTCH1 activity, accelerating myogenicdifferentiation. Positively regulates ASB4 activity, promotingvascular differentiation. Subunit: Homodimer; homodimerization is essential for catalyticactivity. Interacts with VHL and HIF1A. Part of a complex with VHL,HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA.Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4.Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A.Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4(By similarity). Interacts with UBE3A. Subcellular Location: Nucleus. Cytoplasm. Cytoplasm, perinuclearregion. Note=Mainly cytoplasmic localization, but interaction withNOTCH1 results in nuclear localization and interaction with ABPA3results in perinuclear localization in macrophages. Similarity: Contains 1 JmjC domain. Database links: Clone ID: BC007719 Human Entrez Gene: 55662 Human IMAGE Id: 4138066 Human Omim: 606615 Human SwissProt: Q5W147 Human SwissProt: Q9NWT6 Human Unigene: 500788 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
