| 产品编号 | bs-12439R-PE-Cy5.5 |
| 英文名称 | Anti-GCF2/LRRFIP1/PE-Cy5.5 |
| 中文名称 | PE-Cy5.5标记的巨噬细胞调控相关蛋白LRRFIP1抗体 |
| 别 名 | FLAP 1; FLAP1; FLIIAP 1; FLIIAP1; GC binding factor 2; GC-binding factor 2; GCF 2; GCF2; HUFI 1; HUFI1; Leucine rich repeat (in FLII) interacting protein 1; Leucine rich repeat flightless interacting protein 1; Leucine-rich repeat flightless-interacting protein 1; LRR FLII interacting protein 1; LRR FLII-interacting protein 1; LRRF1_HUMAN; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 转录调节因子 结合蛋白 细胞骨架 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 89kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LRRFIP1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: LRRFIP1 is an 738 amino acid human protein whose rodent counterpart is known as Lrrfip1 (also designated FLAP in mouse). LRRFIP1 is also believed to control smooth muscle cell proliferation following arterial injury through PDGF-A repression. The N-terminus of LRRFIP1 shows high homology to the coiled-coil domain of FLAP, a protein which binds the leucine-rich repeat (LRR) of Flightless I, and the interaction of LRRFIP1 with the LRR of Flightless I has been confirmed. LRRFIP1 does not bind single-stranded DNA or RNA significantly and binds double-stranded DNA weakly. In contrast, LRRFIP1 binds double-stranded RNA with high affinity, and two molecules of LRRFIP1 bind the TaR stem. The RNA binding domain has been identified and encompasses a lysine-rich motif. Flightless I has a C-terminal TaR-like domain which binds Actin and therefore the association of LRRFIP1 with the LRR of Flightless I may provide a link between the Actin cytoskeleton and RNA in mammalian cells. Function: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Subunit: Interacts with FLII. Interacts with MYD88. Competes with FLII for MyD88-binding, even in the absence of LPS. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the LRRFIP family. Database links: Entrez Gene: 100066467 Horse Entrez Gene: 9208 Human Entrez Gene: 16978 Mouse Omim: 603256 Human SwissProt: Q32MZ4 Human SwissProt: Q3UZ39 Mouse Unigene: 471779 Human Unigene: 395990 Mouse Unigene: 45039 Mouse Unigene: 153995 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
