产品编号 | bs-13054R-Cy5 |
英文名称 | Anti-eEF1A2/Cy5 |
中文名称 | Cy5标记的翻译延伸因子EF-1 α2抗体 |
别 名 | eEF1A 2; eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 肿瘤 心血管 细胞生物 神经生物学 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, (predicted: Human, Rat, Zebrafish, ) |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 50kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human eEF1A2 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Elongation factor 1 alpha 2 (EF-1 alpha2) is a eukaryotic protein translation factor that is expressed in terminally differentiated cells of skeletal muscle, heart, and certain areas of the brain. Along with protein synthesis, EF-1 alpha2 also plays a role in cytoskeletal remodelling and apoptosis. The gene encoding for EF-1 alpha2 maps to chromosome 20q13. Wasted (wst) refers to a spontaneous autosomal recessive mutation in which this gene is deleted, and it leads to tremors and disturbances of gait shortly after weaning, followed by motor neuron degeneration, paralysis, and death by about 28 days in mice. EF-1 alpha2 has the ability to transform mammalian cells and is highly expressed in tumors of the ovary, breast, and lung, thereby proving a good candidate for an oncogene. In addition to this, EF-1 alpha2 enhances focus formation, allows anchorage-independent growth, and decreases the doubling time of rodent fibroblasts. Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subunit: Monomer Subcellular Location: Nucleus. Tissue Specificity: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. Database links: Entrez Gene: 1917 Human Entrez Gene: 13628 Mouse Entrez Gene: 101109898 Sheep Entrez Gene: 380550 Xenopus laevis Omim: 602959 Human SwissProt: Q05639 Human SwissProt: P62631 Mouse Unigene: 433839 Human Unigene: 2645 Mouse Unigene: 9764 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |