产品编号 | bs-7356R-RBITC |
英文名称 | Anti-2 Cys Peroxiredoxin/RBITC |
中文名称 | 罗丹明(RBITC)标记的硫氧还蛋白还原酶抗体 |
别 名 | Antioxidant enzyme; Peroxiredoxin 1; Peroxiredoxin 2; Peroxiredoxin 3; Peroxiredoxin 4; Peroxiredoxin 5; Peroxiredoxin 5 mitochondrial; PLP; PMP20; PRDX1; PRDX2; PRDX3; PRDX4; PRDX5; PRX V; Thioredoxin reductase. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, (predicted: Mouse, Rat, Pig, Horse, ) |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 17kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human 2 Cys Peroxiredoxin |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Peroxiredoxin (Prx) is a growing peroxidase family whose mammalian members are involved in cell proliferation, differentiation, and apoptosis. There are many isoforms(about 50 proteins), known on the basis of amino acid sequence homology, particularly the amino-terminal region containing an active site cysteine residue. The thiol-specific antioxidant activity is distributed throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2 Cys, atypical 2 Cys Prx, and 1 Cys Prx. Except PrxVI belonging to the 1 Cys Prx subgroup, the other five 2 Cys Prx isotypes share the thioredoxin dependent peroxidase(TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20–30 kilodaltons in molecular size and vary in subcellular localization:PrxI, II and VI in cytosol, PrxIII in mitochondria, PrxIV in ER and secretion, PrxV showing complicated distribution including peroxisome, mitochondria and cytosol. Subcellular Location: Cytoplasmic. Database links: Entrez Gene: 10549 Human Entrez Gene: 10935 Human Entrez Gene: 5052 Human Entrez Gene: 7001 Human SwissProt: P30044 Human SwissProt: P30048 Human SwissProt: P32119 Human SwissProt: Q06830 Human SwissProt: Q13162 Human Unigene: 502823 Human Unigene: 2944 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |