| 产品编号 | bs-14546R-BF594 |
| 英文名称 | Anti-eIF3s8/BF594 |
| 中文名称 | BF594标记的eIF3s8蛋白抗体 |
| 别 名 | Cell migration inducing protein 17; eIF 3c; EIF3 p110; eIF3-p110; eIF3c; eIF3c; EIF3C_HUMAN; EIF3CL; EIF3S8; EIF3S8 protein; Eukaryotic translation initiation factor 3 subunit 8 (110kD); Eukaryotic translation initiation factor 3 subunit 8 110kDa; Eukaryotic translation initiation factor 3 subunit 8; Eukaryotic translation initiation factor 3 subunit c. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 转运蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 105kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eIF3s8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts (1,2). Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation (1-3). Eukaryotic initiation factor-3 (eIF3) is the largest family of eIFs and consists of at least 10 unique subunits (p170, p116, p110, p66, p48, p47, p44, p40, p36, and p35) in mammals (3,4). eIF3 subunit-9 (eIF3-h, eIF3-p116, p116, eIF3-S9, PRT1) is a 873 amino acid component of the eIF3 multi-subunit complex that is involved in ribosomal 48S complex formation (3,5). Association of the eIF3 complex with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits (6,7). Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Subunit: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts with ALKBH4, IFIT1 and IFIT2. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated. Phosphorylation is enhanced upon serum stimulation. Similarity: Belongs to the eIF-3 subunit C family. Contains 1 PCI domain. Database links: Entrez Gene: 8663 Human Entrez Gene: 56347 Mouse Omim: 603916 Human SwissProt: Q99613 Human SwissProt: Q8R1B4 Mouse Unigene: 567374 Human Unigene: 22776 Mouse Unigene: 4207 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
