| 产品编号 | bs-14560R-Cy5.5 |
| 英文名称 | Anti-EIF4EL3/Cy5.5 |
| 中文名称 | Cy5.5标记的EIF4EL3蛋白抗体 |
| 别 名 | 4E LP; 4EHP; 4ELP; EIF 4E type 2; eIF-4E type 2; EIF4 E2; EIF4E 2; EIF4E HOMOLOGOUS PROTEIN; EIF4E like cap binding protein; EIF4E like protein 4E LP; EIF4E like protein 4ELP; EIF4E type 2; EIF4E type 3; eIF4E-like protein 4E-LP; EIF4E2; Eukaryotic translation initiation factor 4E family member 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E like 3; Eukaryotic translation initiation factor 4E member 2; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; IF 4e; IF4E; IF4E2_HUMAN; mRNA cap binding protein 4EHP; mRNA cap binding protein type 3; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EIF4EL3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation factor 4E family (eIF4E) is comprised of three proteins that are involved in the early initiation of protein synthesis. eIF4E2 (eukaryotic translation initiation factor 4E family member 2), also known as 4EHP, IF4e, 4E-LP or EIF4EL3, is a ubiquitously expressed 245 amino acid protein. During early translation events, eIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap (a cotranscriptionally added structure that conveys mRNA stability and allows for efficient RNA processing), thus initiating the unwinding of mRNA secondary structures and facilitating mRNA-ribosome binding. eIF4E2 competes with eIF4E (member 1) for cap binding and, upon modification by the ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein), exhibits increased mRNA cap affinity. Function: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Post-translational modifications: Ubiquitinated by ARIH1, leading to its degradation by the proteasome. Similarity: Belongs to the eukaryotic initiation factor 4E family. Database links: Entrez Gene: 9470 Human Entrez Gene: 26987 Mouse Omim: 605895 Human SwissProt: O60573 Human SwissProt: Q8BMB3 Mouse Unigene: 292026 Human Unigene: 700929 Human Unigene: 227183 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
