产品编号 | bs-14596R-AP |
英文名称 | Anti-ENO4/AP |
中文名称 | 碱性磷酸酶(AP)标记的ENO4蛋白抗体 |
别 名 | C10orf134; AC023283.3; C10orf134; eno4; ENO4_HUMAN; Enolase 4; Enolase family member 4; Enolase-like protein ENO4. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 信号转导 新陈代谢 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat, (predicted: Human, Cow, Rabbit, Sheep, ) |
产品应用 | IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 69kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ENO4 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, alpha enolase, beta enolase and gamma enolase. alpha enolase expression has been detected on most tissues, whereas betaenolase is expressed predominantly in muscle tissue and gamma enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. ENO4 (Enolase-like protein ENO4), also known as C10orf134, is a 628 amino acid protein that belongs to the Enolase family. ENO4 is expressed as two isoforms produced by alternative splicing and is encoded by a gene mapping to human chromosome 10q25.3 and murine chromosome 19 D3. Similarity: Belongs to the enolase family. Database links: Entrez Gene: 387712 Human Entrez Gene: 226265 Mouse Omim: 131375 Human SwissProt: A6NNW6 Human SwissProt: Q8C042 Mouse Unigene: 696001 Human Unigene: 103154 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |