| 产品编号 | bs-18203R-RBITC |
| 英文名称 | Anti-LDB2/RBITC |
| 中文名称 | 罗丹明（RBITC）标记的LDB2蛋白抗体 |
| 别 名 | Carboxyl terminal LIM domain binding protein 1; Carboxyl terminal LIM domain protein 1; Carboxyl-terminal LIM domain-binding protein 1; CLIM 1; CLIM-1; CLIM1; LDB 1; LDB 2; LDB-2; LDB1; LDB2; LDB2_HUMAN; LIM binding domain 2; LIM domain binding 2; LIM domain binding factor 2; LIM domain binding factor CLIM 1; LIM domain binding factor CLIM1; LIM domain binding protein 2; LIM domain-binding factor CLIM1; LIM domain-binding protein 2; OTTHUMP00000158632; OTTHUMP00000217823; OTTHUMP00000217824; OTTHUMP00000217825; OTTHUMP00000217827. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Cow, Horse, Rabbit, Cat, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 43kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LDB2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.[supplied by OMIM, Jul 2010] Function: Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. Subcellular Location: Nucleus. Post-translational modifications: Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Similarity: Belongs to the LDB family. Database links: Entrez Gene: 9079 Human Entrez Gene: 16826 Mouse Omim: 603450 Human SwissProt: Q9W676 Chicken SwissProt: O43679 Human SwissProt: O55203 Mouse Unigene: 714330 Human Unigene: 25785 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
