| 产品编号 | bs-18615R-AP |
| 英文名称 | Anti-MAFG/AP |
| 中文名称 | 碱性磷酸酶（AP）标记的转录因子MAFG抗体 |
| 别 名 | AA545192; Basic leucine zipper transcription factor MafG; C630022N07Rik; hMAF; MAFG; MAFG v maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian); MAFG_HUMAN; MGC13090; MGC20149; RP23 84C12.9; Transcription factor MafG; V maf musculoaponeurotic fibrosarcoma oncogene homolog G; V-maf musculoaponeurotic fibrosarcoma oncogene homolog G. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAFG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010] Function: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in skeletal muscle. Also expressed in heart and brain. Post-translational modifications: Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2. Sumoylation at Lys-14 is required for active transcriptional repression. Similarity: Belongs to the bZIP family. Maf subfamily. Contains 1 bZIP domain. Database links: Entrez Gene: 4097 Human Entrez Gene: 17134 Mouse Omim: 602020 Human SwissProt: O15525 Human SwissProt: O54790 Mouse Unigene: 252229 Human Unigene: 268010 Mouse Unigene: 292957 Mouse Unigene: 393664 Mouse Unigene: 17361 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
