| 产品编号 | bs-18931R-Cy5.5 |
| 英文名称 | Anti-MIA40/Cy5.5 |
| 中文名称 | Cy5.5标记的MIA40蛋白抗体 |
| 别 名 | CHCHD4; Coiled coil helix coiled coil helix domain containing 4; Coiled coil helix coiled coil helix domain containing protein 4; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; FLJ31709; MIA40_HUMAN; Mitochondrial intermembrane space import and assembly 40 homolog; Mitochondrial intermembrane space import and assembly protein 40; TIMM40; Translocase of inner mitochondrial membrane 40 homolog. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 16kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIA40 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008] Function: Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Subcellular Location: Mitochondrion intermembrane space. Tissue Specificity: Expressed in all tissues tested, suggesting an ubiquitous expression. Post-translational modifications: Forms intrachain disulfide bridges, but exists in different redox states. Database links: Entrez Gene: 131474 Human Entrez Gene: 72170 Mouse Entrez Gene: 445144 Zebrafish Omim: 611077 Human SwissProt: Q8N4Q1 Human SwissProt: Q8VEA4 Mouse SwissProt: Q6DEI8 Zebrafish Unigene: 517815 Human Unigene: 41622 Mouse Unigene: 16427 Rat Unigene: 117015 Zebrafish Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
