| 产品编号 | bs-12751R-BF594 |
| 英文名称 | Anti-POFUT2/BF594 |
| 中文名称 | BF594标记的蛋白O岩藻糖基转移酶2抗体 |
| 别 名 | C21orf80; EC 2.4.1.221; FUT 13; FUT13; GDP fucose protein O fucosyltransferase 2; GDP-fucose protein O-fucosyltransferase 2; KIAA0958; O FucT 2; O-FucT-2; OFucT2; OFUT2_HUMAN; Peptide O fucosyltransferase 2; Peptide-O-fucosyltransferase 2; POFUT 2; POFUT2; Protein O fucosyltransferase 2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25, 50kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POFUT2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] Function: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Subcellular Location: Endoplasmic reticulum. Tissue Specificity: Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart. Similarity: Belongs to the glycosyltransferase 68 family. Database links: Entrez Gene: 31098 Fruit fly (Drosophila melanogaster) Entrez Gene: 23275 Human Entrez Gene: 80294 Mouse Omim: 610249 Human SwissProt: Q9W589 Fruit fly (Drosophila melanogaster) SwissProt: Q9Y2G5 Human SwissProt: Q8VHI3 Mouse Unigene: 4643 Fruit fly (Drosophila melanogaster) Unigene: 592164 Human Unigene: 203556 Mouse Unigene: 11702 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
